Effects of Post Ingestion and Physical Conditions on PCR

Amplification of Host Blood Meal DNA in Mosquitoes
*MA Oshaghi 1, AR Chavshin 1, H Vatandoost 1, F Yaaghoobi 1, F Mohtarami 1
M Hashemzadeh 2, N Noorjah 1, MH Modaresi 3
1Dept. of Medical Entomology, School of Public Health and Institute of Health Researches, Tehran University of
Medical Sciences, Iran
2Cellular and Molecular Research Center, Shahre Kord, Charmahal and Bakhtiary University of Medical
Sciences, Iran
3 Dept. of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Iran
(Received 3 Dec 2004; revised 3 Sep 2005; accepted 24 Sep 2005)

Abstract
Identification of host blood meal in haematophagous arthropods is an important element in their rule in transmission of
vector borne diseases. The effects of post ingestion and physical conditions that killed mosquitoes are stored on the success
of detecting blood meal DNA of Anopheles stephensi and Culex quinquefasiatus was investigated by polymerase chain reaction
(PCR) amplification at the human mitochondrial DNA cytochromeB (CytB) gene. Host DNA extracted from the
blood meal up to 33 h post ingestion in both species acts as an efficient template for PCR amplification. However more
DNA concentration needs for meals digested longer time. Successful PCR amplification among meals digested for 36 h
dropping to a faint band. There were no differences between PCR success rate for sampled stored at +4° C or -20° C, but
less successful products were observed in samples kept at 4° C for periods longer than 30 h digestion. The results of this
study is important in malaria epidemiological studies to provide valuable information about the degree of contact between
human hosts and mosquito vectors, impact of vectors controls such bed nets and repellents, and the transmission dynamics
of human malaria and other vector-borne diseases.
Key words: Blood meal, Anopheles stephensi, Culex quinquefasiatus, CytochromeB, Malaria, Vector-borne diseases, Iran

 

Pattern of factor VIII inhibitors in patients with hemophilia A in the north east of Iran

Authors: Modaresi, A. R.1; Mansouri Torghabeh, H.2; Pourfathollah, A. A.2; Mahmoodian Shooshtari, M.3; Rezaie Yazdi, Z.4

Source: Hematology, Volume 11, Number 3, June 2006, pp. 215-217(3)

Publisher: Taylor and Francis Ltd previous article

|View Table of Contents

Key: - Free content - New Content - Subscribed Content - Free Trial Content

Abstract:

This survey was conducted to evaluate coagulation factor VIII:C inhibitors among 102 hemophilia A patients from different cities of Khorasan province in north east of Iran in order to identify and characterize the pattern of inhibitor formation in these patients population. For this purpose, we randomly obtained plasma samples of 102 hemophilia A patients (44 patients with severe, 28 patients with intermediate and 30 patients with mild hemophilia A) and studied them using two tests: the APTT mix and Bethesda test were performed. In the whole group 20 patients (19.6%) factor VIII inhibitors were detected. These were in 11 patients with severe, five patients with intermediate and four patients with mild hemophilia A. None of patients with hemophilia A had previously been studied for the presence of an inhibitor, so there was no existing history of inhibitor evaluation.

Keywords: Hemophilia A; factor VIII inhibitor; Khorasan province; Iran

Document Type: Research article

DOI: 10.1080/10245330600667526

Affiliations: 1: Pediatric Hematology Group, Dr Sheikh Hospital, Mashad, Iran 2: Hematology Group, Medical Science School, Tarbiat Modarres University, Tehran, Iran 3: Blood Transfusion Organization, Tehran, Iran 4: Internal ward of Ghaem Hospital, Masshad, Iran

 


Editorial Manager(tm) for Journal of Pediatric Hematology and Oncology Manuscript Draft Manuscript Number: JPHO 06-270

Title: Renal Splenic Heterotopia with Extramedullary Hematopoiesis in a Thalassemic Patient, Simulating Renal Neoplasm :a case Report
Article Type: Clinical and Laboratory Observations Section/Category:

Keywords: Key words: Spleen-Renal- Thallasemia- Heterotopia- neoplasm
Corresponding Author: Dr mehran hiradfar, M.D.
Corresponding Author's Institution: Dr sheikh pediatric Hospital
First Author: Mehran Hiradfar, M.D.
Order of Authors: Mehran Hiradfar, M.D.; Mehran Hiradfar, M.D.; mehran hiradfar, M.D.; Nona Zabolinejad,
M.D.; abdolla Banihashem, M.D.; Mehran Hiradfar, M.D.; Nona Zabolinejad, M.D.; Abdollah Banihashem,
M.D.; mehran Hiradfar, M.D.; Nona Zabolinejad, M.D.; Abdollah Banihashem, M.D.
Manuscript Region of Origin: IRAN, ISLAMIC REPUBLIC OF

Abstract: Abstract
Intrarenal splenic tissue (splenorenal heterotopia)(SRH) is a rare condition . Simultaneous existence of SRH
and extramedullary hematopoiesis in a same patient can present as a mass of kidney .
We present an intresting case of splenorenal hetrotopia with EMH in a thallasemic child simulating as a
renal neoplasm .This is the first reported case in the right kidney.


Diagnostic Value of Ultrasound Findings in Mesenteric Lymphadenitis in Children with Acute Abdominal Pain

Backgrounds/Objective: The aim of this study was to compare abdominal sonographic features of patients with mesenteric lymphadenitis versus sonographic features of the asymptomatic children, in order to identify diagnost ic criteria for mesenteric lymphadenitis. Patients and Methods: Ultrasound data from 113 children with acute abdominal pain whose suspected sonographic diagnosis was mesenteric adenitis or ileitis was inspected in a child hospital in March 2004 to March 2005. The fina l diagnoses were established after patient management and follow-up. As for the control group, a hundred asymptomatic children were referred from health centers or kindergartens.

Results: No para-aortic lymphadenopathy was found in any of the subjects from the study and the control groups. The number of enlarge d mesenteric lymph nodes observed in symptomatic children was significantly greater than the figure in asymptomatic children (P 0.0001). The highest rate of sensitivity was seen with the presence of RLQ lymphadenopathy (94.7%) and the highest rate of specifici ty was seen with the presence of lymphadenopathy in two or three areas of LUQ or mi d-abdomen together; lymphadenopathies larger than 6mm in RLQ; presence of more than 4 lymph nodes (with any size) in a sonographic image from RLQ; or thickening of terminal il eum more than 8mm (92%-100%) in the diagnosis
of mesenteric lymphadenitis. Conclusion: In children with acute abdominal pain due to causes such as appendicitis which cannot be confirmed by ultrasound and the f indings consist of only enlarged mesenteric lymph nodes (≥4mm) and/or mural thickening of the terminal ileum ( ≥8mm), the probable diagnosis would be mesenteric adenitis-ileitis and surgery is not required; but close observation is recommended.