Evaluation of Prevalence of MRSA Colonization in 150 Neonates two Medical Centers in Iran

AUTHORS AND SPEAKER:

· Professor M.H. Soltanzaheh, MD,ID,Prof. Of Pediatrics. Shahid Beheshti University of Medical Sciences, Tehran.IRAN.(Speaker)

· Dr A.H. Arbabi, MD Assistant prof, of Iran University Tehran, Iran.

· Dr F.ShirvAni, MD, ID, Assistant prof, of Pediatrics Shahid Beheshti University, Tehran, Iran.

· Dr K. Mirnia, MD Resident. Of Pediatrics. Shahid Beheshti University, Tehran, Iran.

· Dr M. Radfar, MD Assistant prof, Of Pediatrics. Neonatologist, Shahid Beheshti University, Tehran, Iran

· Dr M. Khodami, MD Associate of Pathology, Shahid Beheshti University, Tehran, Iran.

· M. Gharouni. Microbiologist, Shahid Beheshti University, Tehran, Iran.

ABSTRACT

Background: Penicillin was introduced for s-aureus treatment in 1940. However with the over usage this antibiotic, S-aureus has become increasingly Antibiotic Resistant.

Due to this problem we studied the epidemiological prevalence of MRSA colonization on 150 Neonates in two Medical Centers, Imam Hossein and Shahid Akbarabadi.

Methods: Skin and Umbilical samples obtained from 150 newborns in two Medical Centers after72 hours of admission. Also samples were obtained from anterior nasal mucosa of personnel involved with Neonates in that department by sterile cotton swap.

Sterile swap was inoculated in to nutrient agar then within 24 h was transferred to sheep blood agar. Colonies that were gram positive in smear and were coagulase positive and DNAase positive were known as-s-aureus. As s-aureus were transformed to mulleur hinton NaCL 4% culture that had Oxacillin Disk.

After 24 h of incubation at 37 ̊ C, colonies that grew< 13mm around the Disk were known as MRSA, and colonies that grew>13 mm were known as MSSA.

Information related to neonates and personnel were collected by completing a questionnaire. All the data were analyzed by statistical software SPSS-11

Results: In our study, 47/3% of neonates were Male and 52/7% were famale.

Prevalence of MRSA in male was 52/8% and in female 47/2%. Mean weight of newborns were 2394 grams+/- 1119 grams.

61% of S-aureus were Resistant to Oxacillin. Prevalence of MRSA in Imam Hossein Hospital was 96/6% and in Shahid Akbarabadi was 26/7% that this difference was statistically significant P<0.0001. All MRSA were sensitive to Vancomycin in Clindamycin were statistically significant P<0.001.

In Akbarabadi Hospital Difference of MRSA were sensitive to Vancomycin in Imam Hossain Hospital. Difference of MRSA S-Resistant to Cloxacillin and Clindamycin were statistically significant P<0.0001.

Hospital and Mothers were the most effective agent in MRSA S Prevalence.

Conclusion : Imam hossein is a general Hospital including an adult Surgical Ward but Shahid Akbarabadi Hospital is a Delivery Center that has an active NICU.

Prevalence of MRSA in Imam Hossein Hospital is greater than Shahid Akbarabadi Hospital due to more variety of patients and over usage Antibiotics. MRSA colonies resistant Cloxacillin, Clindamycin and Penicillin are more are more in Immam Hossain Hospital and therefore these antibiotics are not used. This Resistance is fewer in Shahid Akbarabadi Hospital and therefore these Antibiotics are still used.